Hydroxyapatite incorporated into collagen gels for mesenchymal stem cell culture.
Fiche publication
Date publication
janvier 2013
Journal
Bio-medical materials and engineering
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DE ISLA Natalia
Tous les auteurs :
Laydi F, Rahouadj R, Cauchois G, Stoltz JF, de Isla N
Lien Pubmed
Résumé
Collagen gels could be used as carriers in tissue engineering to improve cell retention and distribution in the defect. In other respect hydroxyapatite could be added to gels to improve mechanical properties and regulate gel contraction. The aim of this work was to analyze the feasibility to incorporate hydroxyapatite into collagen gels and culture mesenchymal stem cells inside it. Human bone marrow mesenchymal stem cells (hMSC-BM) were used in this study. Gels were prepared by mixing rat tail type I collagen, hydroxyapatite microparticles and MSCs. After polymerization gels were kept in culture while gel contraction and mechanical properties were studied. In parallel, cell viability and morphology were analyzed. Gels became free-floating gels contracted from day 3, only in the presence of cells. A linear rapid contraction phase was observed until day 7, then a very slow contraction phase took place. The incorporation of hydroxyapatite improved gel stability and mechanical properties. Cells were randomly distributed on the gel and a few dead cells were observed all over the experiment. This study shows the feasibility and biocompatibility of hydroxyapatite supplemented collagen gels for the culture of mesenchymal stem cells that could be used as scaffolds for cell delivery in osteoarticular regenerative medicine.
Mots clés
Animals, Biocompatible Materials, chemistry, Bone Marrow Cells, physiology, Cell Culture Techniques, Cell Shape, Cell Survival, physiology, Collagen, chemistry, Collagen Type I, chemistry, Durapatite, chemistry, Feasibility Studies, Humans, Hydrogels, chemistry, Mechanical Phenomena, Mesenchymal Stromal Cells, physiology, Particle Size, Polymerization, Rats, Surface Properties, Time Factors, Tissue Engineering, Tissue Scaffolds, chemistry
Référence
Biomed Mater Eng. 2013 ;23(4):311-5