Using correlative light and electron microscopy to study zebrafish vascular morphogenesis.
Fiche publication
Date publication
janvier 2015
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GOETZ Jacky
Tous les auteurs :
Goetz JG, Monduc F, Schwab Y, Vermot J
Lien Pubmed
Résumé
Live imaging is extremely useful to characterize the dynamics of cellular events in vivo, yet it is limited in terms of spatial resolution. Correlative light and electron microscopy (CLEM) allows combining live confocal microscopy with electron microscopy (EM) for the characterization of biological samples at high temporal and spatial resolution. Here we describe a protocol allowing extracting endothelial cell ultrastructure after having imaged the same cell in its in vivo context through live confocal imaging during zebrafish embryonic development.
Mots clés
Anesthesia, Animals, Blood Vessels, embryology, Embryo, Nonmammalian, ultrastructure, Fluorescence, Image Processing, Computer-Assisted, Lasers, Microscopy, Electron, methods, Morphogenesis, Resins, Synthetic, Tissue Fixation, Tomography, Zebrafish, embryology
Référence
Methods Mol. Biol.. 2015 ;1189:31-46