Stem-loop RT-PCR based quantification of small non-coding RNAs.
Fiche publication
Date publication
janvier 2015
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr REDERSTORFF Mathieu
Tous les auteurs :
Salone V, Rederstorff M
Lien Pubmed
Résumé
Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse-transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.
Mots clés
DNA Primers, genetics, Inverted Repeat Sequences, genetics, RNA, Small Untranslated, analysis, Real-Time Polymerase Chain Reaction, methods
Référence
Methods Mol. Biol.. 2015 ;1296:103-8