In vitro RNA uptake studies in plant mitochondria.
Fiche publication
Date publication
janvier 2015
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MARÉCHAL-DROUARD Laurence
Tous les auteurs :
Kubiszewski-Jakubiak S, Megel C, Ubrig E, Salinas T, Duchêne AM, Maréchal-Drouard L
Lien Pubmed
Résumé
During evolution, most of the ancestral genes from the endosymbiotic α-proteobacteria at the origin of mitochondria have been either lost or transferred to the nuclear genome. To allow the comeback of proteins and RNAs [in particular transfer RNA (tRNAs)] into the organelle, macromolecule import systems were universally established. While protein import processes have been studied into details, much less is known about tRNA mitochondrial import. In plants, part of the knowledge on the tRNA import process into mitochondria has been acquired thanks to in vitro import assays. Furthermore, the development of in vitro RNA import strategies allowed the study of plant mitochondrial gene expression. The purpose of this chapter is to provide detailed protocols to perform in vitro RNA uptake into potato (Solanum tuberosum) or Arabidopsis (Arabidopsis thaliana) mitochondria as well as approaches to analyze them.
Mots clés
Arabidopsis, genetics, Electrophoresis, Polyacrylamide Gel, methods, Mitochondria, genetics, RNA Transport, RNA, Plant, genetics, RNA, Transfer, genetics, Reverse Transcriptase Polymerase Chain Reaction, methods, Solanum tuberosum, genetics, Transcription, Genetic
Référence
Methods Mol. Biol.. 2015 ;1305:45-60