Gene expression knockdown by transfection of siRNAs into mammalian cells.
Fiche publication
Date publication
janvier 2015
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr REDERSTORFF Mathieu
Tous les auteurs :
Abel Y, Rederstorff M
Lien Pubmed
Résumé
SiRNA induced gene silencing or RNA interference is a powerful tool to knock down gene expression and perform gene function studies. In this chapter, we describe a basic method to silence gene expression by transfecting a specific synthetic siRNA into mammalian HeLa cells.
Mots clés
HeLa Cells, Humans, RNA Interference, RNA, Small Interfering, genetics, Synthetic Biology, methods, Transfection, methods
Référence
Methods Mol. Biol.. 2015 ;1296:199-202