Construction of biocompatible porous tissue scaffold from the decellularized umbilical artery.
Fiche publication
Date publication
janvier 2015
Journal
Bio-medical materials and engineering
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DECOT Véronique
Tous les auteurs :
Xin Y, Wu G, Wu M, Zhang X, Velot E, Decot V, Cui W, Huang Y, Stoltz JF, Du J, Li N
Lien Pubmed
Résumé
The scaffolds prepared from the tissue decellularization conserve the porous 3-D structure and provide an optimal matrix for the tissue regeneration. Since decade, the enzymatic digestion, chemical reagent treatment and mechanical actions such as eversion and abrasion have been used to remove the cells from the intact matrix. In this study, we optimized an enzymatic method to decellularize the umbilical artery to construct a 3-D porous scaffold which is suitable for the culture of mesenchymal stem cells (MSCs). The scaffold maintained the interconnected porous structure. It remained the similar high water content 95.3 ± 1% compared to 94.9 ± 0.6% in the intact umbilical artery (p>0.05). The decellularization process decreased the stress from 0.24 ± 0.05 mPa to 0.15 ± 0.06 mPa (p<0.05). However the decellularization did not change the strain of the artery (45 ± 15% vs. 53 ± 10%, p>0.05). When the scaffold was transplanted to the subcutaneous tissue in the wild type mice, there were less T cells appeared in the surrounding tissue which meant the decreased the immunogenicity by decellularization. This scaffold also supported the adhesion and proliferation of the MSCs. In this study, we constructed a biological compatible porous scaffold from the decellularized umbilical artery which may provide a suitable scaffold for cell-matrix interaction studies and for tissue engineering.
Mots clés
Animals, Biocompatible Materials, chemical synthesis, Cell Adhesion, physiology, Cell Proliferation, physiology, Cell-Free System, Cells, Cultured, Compressive Strength, Elastic Modulus, Equipment Design, Equipment Failure Analysis, Extracellular Matrix, chemistry, Humans, Materials Testing, Mesenchymal Stromal Cells, cytology, Mice, Porosity, Tensile Strength, Tissue Engineering, instrumentation, Tissue Scaffolds, Umbilical Arteries, chemistry
Référence
Biomed Mater Eng. 2015 ;25(1 Suppl):65-71