Two-photon-induced Förster resonance energy transfer in a hybrid material engineered from quantum dots and bacteriorhodopsin.
Fiche publication
Date publication
avril 2015
Journal
Optics letters
Auteurs
Membres identifiés du Cancéropôle Est :
Pr NABIEV Igor
Tous les auteurs :
Krivenkov V, Samokhvalov P, Solovyeva D, Bilan R, Chistyakov A, Nabiev I
Lien Pubmed
Résumé
Energy transfer from nanostructures to biological supramolecular photosystems is an important fundamental issue related to the possible influence of nanoobjects on biological functions. We demonstrate here two-photon-induced Förster resonance energy transfer (FRET) from fluorescent CdSe/ZnS quantum dots (QDs) to the photosensitive protein bacteriorhodopsin (bR) in a QD-bR hybrid material. The two-photon absorption cross section of QDs has been found to be about two orders of magnitude larger than that of bR. Therefore, highly selective two-photon excitation of QDs in QD-bR complexes is possible. Moreover, the efficiency of FRET from QDs to bR is sufficient to initiate bR photoconversion through two-photon excitation of QDs in the infrared spectral region. The data demonstrate that the effective spectral range in which the bR biological function is excited can be extended beyond the band where the protein itself utilizes light energy, which could open new ways to use this promising biotechnological material.
Mots clés
Bacteriorhodopsins, chemistry, Cadmium Compounds, chemistry, Fluorescence Resonance Energy Transfer, methods, Photons, Quantum Dots, chemistry, Selenium Compounds, chemistry, Sulfides, chemistry, Zinc Compounds, chemistry
Référence
Opt Lett. 2015 Apr 1;40(7):1440-3