A Prokineticin-Driven Epigenetic Switch Regulates Human Epicardial Cell Stemness and Fate.
Fiche publication
Date publication
octobre 2018
Journal
Stem cells (Dayton, Ohio)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr NEBIGIL-DESAUBRY Canan
Tous les auteurs :
Qureshi R, Kindo M, Boulberdaa M, von Hunolstein JJ, Steenman M, Nebigil CG
Lien Pubmed
Résumé
Epicardial adipose tissues (EATs) and vascular tissues may both belong to the mesoepithelial lineage that develops from epicardium-derived progenitor cells (EPDCs) in developing and injured hearts. Very little is known of the molecular mechanisms of EPDC contribution in EAT development and neovascularization in adult heart, which the topic remains a subject of intense therapeutic interest and scientific debate. Here we studied the epigenetic control of stemness and anti-adipogenic and pro-vasculogenic fate of human EPDCs (hEPDCs), through investigating an angiogenic hormone, prokineticin-2 (PK2) signaling via its receptor PKR1. We found that hEPDCs spontaneously undergoes epithelial-to-mesenchymal transformation (EMT), and are not predestined for the vascular lineages. However, PK2 via a histone demethylase KDM6A inhibits EMT, and induces asymmetric division, leading to self-renewal and formation of vascular and epithelial/endothelial precursors with angiogenic potential capable of differentiating into vascular smooth muscle and endothelial cells. PK2 upregulates and activates KDM6A to inhibit repressive histone H3K27me3 marks on promoters of vascular genes (Flk-1 and SM22α) involved in vascular lineage commitment and maturation. In PK2-mediated anti-adipogenic signaling, KDM6A stabilizes and increases cytoplasmic β-catenin levels to repress peroxisome proliferator-activated receptor-γ expression and activity. Our findings offer additional molecular targets to manipulate hEPDCs-involved tissue repair/regeneration in cardiometabolic and ischemic heart diseases. Stem Cells 2018;36:1589-1602.
Mots clés
Angiogenesis, Asymmetric division, Differentiation, Epicardial adipose tissue, Epicardial to mesenchymal transformation, Epicardial-derived progenitor cells, H3K27me3, KDM6A/UTX, Prokineticin, Self-renewal, Vascular smooth muscle cells
Référence
Stem Cells. 2018 10;36(10):1589-1602