A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening.
Fiche publication
Date publication
avril 2015
Journal
Biomaterials science
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Neto AI, Correia CR, Oliveira MB, Rial-Hermida MI, Alvarez-Lorenzo C, Reis RL, Mano JF
Lien Pubmed
Résumé
We propose a novel hanging spherical drop system for anchoring arrays of droplets of cell suspension based on the use of biomimetic superhydrophobic flat substrates, with controlled positional adhesion and minimum contact with a solid substrate. By facing down the platform, it was possible to generate independent spheroid bodies in a high throughput manner, in order to mimic in vivo tumour models on the lab-on-chip scale. To validate this system for drug screening purposes, the toxicity of the anti-cancer drug doxorubicin in cell spheroids was tested and compared to cells in 2D culture. The advantages presented by this platform, such as feasibility of the system and the ability to control the size uniformity of the spheroid, emphasize its potential to be used as a new low cost toolbox for high-throughput drug screening and in cell or tissue engineering.
Mots clés
Antineoplastic Agents, chemistry, Biocompatible Materials, chemistry, Biomimetics, Biotechnology, Cell Culture Techniques, Cell Line, Tumor, Cell Survival, drug effects, Doxorubicin, chemistry, Drug Evaluation, Preclinical, instrumentation, High-Throughput Screening Assays, methods, Humans, Spheroids, Cellular, cytology, Tissue Engineering, methods
Référence
Biomater Sci. 2015 Apr;3(4):581-5