Quantum dot surface chemistry and functionalization for cell targeting and imaging.
Fiche publication
Date publication
avril 2015
Journal
Bioconjugate chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Pr NABIEV Igor
Tous les auteurs :
Bilan R, Fleury F, Nabiev I, Sukhanova A
Lien Pubmed
Résumé
Quantum dots (QDs) are highly fluorescent nanoscale crystals with size-dependent emission spectra. Due to their excellent photophysical properties, QDs are a promising alternative to organic fluorescent dyes and fluorescent proteins for cell targeting, imaging, and drug delivery. For biomedical applications, QDs should be chemically modified to be stable in aqueous solutions and tagged with the recognition molecules or drugs. Here, we review surface modification approaches to, and strategies for, conjugation of bioactive molecules with QDs. There are a variety of methods of QD surface modification and QD incorporation into larger delivery systems that yield fluorescent nanocarriers from 10 nm to several micrometers. Conjugates of QDs with peptides, proteins, antibodies, oligonucleotides, and small molecules have been used for fluorescent targeting, tracking, and imaging both in vitro and in vivo. Due to an extremely high stability to photobleaching, QDs were used for long-term visualization. QD applications pave the way for new generations of ultrasensitive detection, diagnostic systems, as well as drug delivery approaches, combining accurate targeting, delivery, and imaging in a single assay.
Mots clés
Animals, Antibodies, chemistry, Cell Tracking, methods, Drug Carriers, chemical synthesis, Eukaryotic Cells, drug effects, Humans, Immunoconjugates, chemistry, Molecular Imaging, methods, Molecular Probes, chemical synthesis, Nanoparticles, chemistry, Nucleic Acids, chemistry, Particle Size, Peptides, chemistry, Quantum Dots, chemistry, Staining and Labeling, methods, Surface Properties
Référence
Bioconjug. Chem.. 2015 Apr 15;26(4):609-24