Dielectrophoretic Traps for Efficient Bead and Cell Trapping and Formation of Aggregates of Controlled Size and Composition.
Fiche publication
Date publication
juillet 2022
Journal
Frontiers in bioengineering and biotechnology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOLOPION Aude, Dr GAUTHIER Michaël
Tous les auteurs :
Lipp C, Koebel L, Bertsch A, Gauthier M, Bolopion A, Renaud P
Lien Pubmed
Résumé
We present a microfluidic dielectrophoretic-actuated system designed to trap chosen single-cell and form controlled cell aggregates. A novel method is proposed to characterize the efficiency of the dielectrophoretic trapping, considering the flow speed but also the heat generated by the traps as limiting criteria in cell-safe manipulation. Two original designs with different manufacturing processes are experimentally compared. The most efficient design is selected and the cell membrane integrity is monitored by fluorescence imaging to guarantee a safe-cell trapping. Design rules are suggested to adapt the traps to multiple-cells trapping and are experimentally validated as we formed aggregates of controlled size and composition with two different types of cells. We provide hereby a simple manufactured tool allowing the controlled manipulation of particles for the composition of multicellular assemblies.
Mots clés
cell trapping, cellular assemblies, dielectrophoreis (DEP), electroporation, microfluidics
Référence
Front Bioeng Biotechnol. 2022 07 14;10:910578