Antibacterial Photodynamic Therapy in the Near-Infrared Region with a Targeting Antimicrobial Peptide Connected to a π-Extended Porphyrin.
Fiche publication
Date publication
juillet 2022
Journal
ACS infectious diseases
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BECHINGER Burkhard, Dr LAVALLE Philippe, Dr RABINEAU Morgane
Tous les auteurs :
Gourlot C, Gosset A, Glattard E, Aisenbrey C, Rangasamy S, Rabineau M, Ouk TS, Sol V, Lavalle P, Gourlaouen C, Ventura B, Bechinger B, Heitz V
Lien Pubmed
Résumé
The increase of antimicrobial resistance to conventional antibiotics is worldwide a major health problem that requires the development of new bactericidal strategies. Antimicrobial photodynamic therapy (a-PDT) that generates reactive oxygen species acting on multiple cellular targets is unlikely to induce bacterial resistance. This localized treatment requires, for safe and efficient treatment of nonsuperficial infections, a targeting photosensitizer excited in the near IR. To this end, a new conjugate consisting of an antimicrobial peptide linked to a π-extended porphyrin photosensitizer was designed for a-PDT. Upon irradiation at 720 nm, the conjugate has shown at micromolar concentration strong bactericidal action on both Gram-positive and Gram-negative bacteria. Moreover, this conjugate allows one to reach a low minimum bactericidal concentration with near IR excitation without inducing toxicity to skin cells.
Mots clés
antimicrobial peptide, antimicrobial photodynamic therapy, bacteria, keratinocyte, near infrared, porphyrin
Référence
ACS Infect Dis. 2022 07 27;: