Impact of the type of microcarrier and agitation modes on the expansion performances of mesenchymal stem cells derived from umbilical cord.
Fiche publication
Date publication
novembre 2019
Journal
Biotechnology progress
Auteurs
Membres identifiés du Cancéropôle Est :
Dr REPPEL Loïc, Dr DE ISLA Natalia
Tous les auteurs :
Loubière C, Sion C, De Isla N, Reppel L, Guedon E, Chevalot I, Olmos E
Lien Pubmed
Résumé
The present study proposed to compare the impact of agitation mode (static, orbital, and mechanical) on the culture of mesenchymal stem cells extracted from the Wharton's jelly of umbilical cords (WJ-MSC), in a clinical grade culture medium, using human platelet lysate and different xeno-free microcarriers. Attachment, expansion, and detachment performances were characterized by a new dedicated tool of microscopic image posttreatment, allowing an in situ cell counting without detachment step. Results showed that performances in static mode were not necessarily representative of those obtained in dynamic mode. Moreover, impacts on nutrient consumptions and metabolite productions were identified, such as a higher glutamine consumption when Cytodex-1 microcarriers were used. The detachment strategy used was relatively efficient for Star-Plus, Plastic-Plus, and Hillex II, but not sufficient for Cytodex-1. Despite Cytodex-1 presented promising attachment and expansion performances, Star-Plus and Plastic-Plus showed a better compromise, respectively, for the orbital and the mechanical agitation modes.
Mots clés
Wharton's jelly, agitation, cell counting method, mesenchymal stem cells, microcarrier
Référence
Biotechnol Prog. 2019 11;35(6):e2887