Mixing Block Copolymers with Phospholipids at the Nanoscale: From Hybrid Polymer/Lipid Wormlike Micelles to Vesicles Presenting Lipid Nanodomains.
Fiche publication
Date publication
février 2017
Journal
Langmuir : the ACS journal of surfaces and colloids
Auteurs
Membres identifiés du Cancéropôle Est :
Dr SCHMUTZ Marc
Tous les auteurs :
Dao TP, Brûlet A, Fernandes F, Er-Rafik M, Ferji K, Schweins R, Chapel JP, Fedorov A, Schmutz M, Prieto M, Sandre O, Le Meins JF
Lien Pubmed
Résumé
Hybrids, i.e., intimately mixed polymer/phospholipid vesicles, can potentially marry in a single membrane the best characteristics of the two separate components. The ability of amphiphilic copolymers and phospholipids to self-assemble into hybrid membranes has been studied until now on the submicrometer scale using optical microscopy on giant hybrid unilamellar vesicles (GHUVs), but limited information is available on large hybrid unilamellar vesicles (LHUVs). In this work, copolymers based on poly(dimethylsiloxane) and poly(ethylene oxide) with different molar masses and architectures (graft, triblock) were associated with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Classical protocols of LUV formation were used to obtain nanosized self-assembled structures. Using small-angle neutron scattering (SANS), time-resolved Förster resonance energy transfer (TR-FRET), and cryo-transmission electron microscopy (cryo-TEM), we show that copolymer architecture and molar mass have direct influences on the formation of hybrid nanostructures that can range from wormlike hybrid micelles to hybrid vesicles presenting small lipid nanodomains.
Mots clés
1,2-Dipalmitoylphosphatidylcholine, chemistry, Dimethylpolysiloxanes, chemistry, Micelles, Models, Molecular, Molecular Conformation, Nanostructures, chemistry, Polyethylene Glycols, chemistry, Unilamellar Liposomes, chemistry
Référence
Langmuir. 2017 02 21;33(7):1705-1715