Development and Validation of Antibodies Targeting Site-Specific Histone Methylation.
Fiche publication
Date publication
janvier 2022
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DAUJAT Sylvain
Tous les auteurs :
Zorro Shahidian L, Daujat S
Lien Pubmed
Résumé
The development of specific anti-modification antibodies as research tools has revolutionized the way histone methylation is studied. Lack of stringent quality controls, however, led to the development of nonspecific antibodies, compromising their use. In this chapter, we provide a series of protocols that collectively will help those studying histone methylation to develop and thoroughly validate high-end sequence-specific and methylation-dependent antibodies.
Mots clés
Affinity purification, Antibody validation, Histone methylation, Rabbit polyclonal antibodies, Site-specific antibody development
Référence
Methods Mol Biol. 2022 ;2529:171-206