Spectroscopic and microscopic characterization of dipicolinic acid and its salt photoproducts - A UVc effect study on DPA in solution and in bacterial spores.
Fiche publication
Date publication
juin 2022
Journal
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Auteurs
Membres identifiés du Cancéropôle Est :
Pr PERRIER-CORNET Jean-Marie
Tous les auteurs :
Pacheco M, Dikec J, Winckler P, Coelho C, Perrier-Cornet JM
Lien Pubmed
Résumé
Bacterial spores can cause significant problems such as food poisoning (like neurotoxin or emetic toxin) or serious illnesses (like anthrax or botulism). This dormant form of bacteria, made of several layers of barriers which provide extreme resistance to many abiotic stresses (radiation, temperature, pressure, etc.), are difficult to investigate in situ. To better understand the biological and chemical mechanisms involved and specific to spores resistance, the acquisition of environmental parameters is necessary. For that purpose, our research has been focused on the detection and analysis of a unique spore component, dipicolinic acid (DPA), used as the main in situ metabolite for sporulating bacteria detection. In its native form, DPA is only weakly fluorescent but after Ultraviolet irradiation at the wavelength of 254 nm (UVc), DPA photoproducts (DPAp) exhibit a remarkable fluorescence signal. These photoproducts are rarely identified and part of this study gives new insights offered by mass spectrometry (MS) in the determination of DPA photoproducts. Thanks to DPA assay techniques and fluorescence spectrometry, we highlighted the instability of photoproducts and introduced new assumptions on the effects of UVc on DPA. Studies in spectroscopy and microscopy allowed us to better understand these native probes in bacterial spores and will allow the implementation of a new method for studying the physico-chemical parameters of spore resistance.
Mots clés
Bacterial spore, DPA, Fluorescence lifetime, Native probe, Phasor plot, Photoproducts, Two-photon microscopy
Référence
Spectrochim Acta A Mol Biomol Spectrosc. 2022 06 17;280:121502