All-Aqueous Freeform Fabrication of Perfusable Self-Standing Soft Compartments.
Fiche publication
Date publication
juin 2022
Journal
Advanced materials (Deerfield Beach, Fla.)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Gonçalves RC, Vilabril S, Neves CMSS, Freire MG, Coutinho JAP, Oliveira MB, Mano JF
Lien Pubmed
Résumé
Compartmentalized structures obtained in all-aqueous settings have shown promising properties as cell encapsulation devices, as well as reactors for trans-membrane chemical reactions. While most approaches focus on the preparation of spherical devices, advances on the production of complex architectures have been enabled by the interfacial stability conferred by emulsion systems, namely mild aqueous two-phase systems (ATPS), or non-equilibrated analogues. However, the application of non-spherical structures has mostly been reported while keeping the fabricated materials at a stable interface, limiting the free-standing character, mobility, and transposition of the obtained structures to different setups. Here, we show the fabrication of self-standing, malleable and perfusable tubular systems through all-aqueous interfacial assembly, culminating in the preparation of independent objects with stability and homogeneity after disruption of the polymer-based aqueous separating system. Those hollow structures could be fabricated with a variety of widths, and rapidly printed as long structures at flow rates of 15 mm s . The materials were used as compartments for cell culture, showcasing high cytocompatibility, and could be tailored to promote cell adhesion. Such structures may find application in fields that benefit from freeform tubular structures, including the biomedical field with, e.g., cell encapsulation, and benchtop preparation of microfluidic devices. This article is protected by copyright. All rights reserved.
Mots clés
all-aqueous fabrication, aqueous two-phase systems, cell encapsulation, interfacial complexation, tubular/hollow materials
Référence
Adv Mater. 2022 06 13;:e2200352