Analysis of Metabolic Pathways by C-Labeled Molecular Probes and HRMAS Nuclear Magnetic Resonance Spectroscopy: Isotopologue Identification and Quantification Methods for Medical Applications.
Fiche publication
Date publication
juin 2022
Journal
Analytical chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Pr NAMER Izzie-Jacques
Tous les auteurs :
Outilaft H, Bund C, Piotto M, Namer IJ
Lien Pubmed
Résumé
The use of C-labeled molecular probes is essential to explore altered metabolic pathways in human pathologies. The analysis of the different C isotopologues resulting from these changes in metabolic pathways is essential to understand the different biological processes involved. We propose an NMR methodology consisting of eight different NMR experiments performed under HRMAS conditions to explore metabolic pathways in unprocessed pathological cells and tissues. This methodology has the potential to study human pathologies in the medical field and to enable the analysis of the mode of action of therapeutic treatments.
Mots clés
Humans, Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy, methods, Metabolic Networks and Pathways, Molecular Probes
Référence
Anal Chem. 2022 06 14;94(23):8226-8233