Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in .
Fiche publication
Date publication
juin 2022
Journal
STAR protocols
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ROMIER Christophe
Tous les auteurs :
Picchi-Constante GFA, Hiraiwa PM, Marek M, Rogerio VZ, Guerra-Slompo EP, Romier C, Zanchin NIT
Lien Pubmed
Résumé
This protocol outlines a new genetic complementation strategy to investigate gene function in , the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene containing the desired mutations that are resistant to Cas9-cleavage, which enables detailed investigation of protein function. This experimental strategy overcomes some of the limitations associated with gene knockouts in . For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).
Mots clés
CRISPR, Cell Biology, Microbiology, Model Organisms, Molecular Biology
Référence
STAR Protoc. 2022 Jun 17;3(2):101324