Polynucleotide differentiation using hybrid solid-state nanopore functionalizing with α-hemolysin.
Fiche publication
Date publication
janvier 2020
Journal
Soft matter
Auteurs
Membres identifiés du Cancéropôle Est :
Dr PICAUD Fabien
Tous les auteurs :
Bentin J, Balme S, Picaud F
Lien Pubmed
Résumé
We report results from full atomistic molecular dynamics simulations on the properties of biomimetic nanopores. This latter result was obtained through the direct insertion of an α-hemolysin protein inside a hydrophobic solid-state nanopore. Upon translocation of different DNA strands, we demonstrate here that the theoretical system presents the same discrimination properties as the experimental one obtained previously. This opens an interesting way to promote the stability of a specific protein inside a solid nanopore to develop further biomimetic applications for DNA or protein sequencing.
Mots clés
Amino Acid Sequence, genetics, Biomimetics, DNA, chemistry, Hemolysin Proteins, chemistry, Hydrophobic and Hydrophilic Interactions, Molecular Dynamics Simulation, Nanopores, ultrastructure, Polynucleotides, chemistry
Référence
Soft Matter. 2020 Jan 28;16(4):1002-1010