Bioengineered Hierarchical Bonelike Compartmentalized Microconstructs Using Nanogrooved Microdiscs.
Fiche publication
Date publication
avril 2022
Journal
ACS applied materials & interfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Bjørge IM, de Sousa BM, Patrício SG, Silva AS, Nogueira LP, Santos LF, Vieira SI, Haugen HJ, Correia CR, Mano JF
Lien Pubmed
Résumé
Fabrication of vascularized large-scale constructs for regenerative medicine remains elusive since most strategies rely solely on cell self-organization or overly control cell positioning, failing to address nutrient diffusion limitations. We propose a modular and hierarchical tissue-engineering strategy to produce bonelike tissues carrying signals to promote prevascularization. In these 3D systems, disc-shaped microcarriers featuring nanogrooved topographical cues guide cell behavior by harnessing mechanotransduction mechanisms. A sequential seeding strategy of adipose-derived stromal cells and endothelial cells is implemented within compartmentalized, liquefied-core macrocapsules in a self-organizing and dynamic system. Importantly, our system autonomously promotes osteogenesis and construct's mineralization while promoting a favorable environment for prevascular-like endothelial organization. Given its modular and self-organizing nature, our strategy may be applied for the fabrication of larger constructs with a highly controlled starting point to be used for local regeneration upon implantation or as drug-screening platforms.
Mots clés
biophysical cues, bottom-up tissue engineering, cell self-assembly, compartmentalization, liquefied-core capsules, nanogrooved microdiscs, osteogenesis, prevascularization
Référence
ACS Appl Mater Interfaces. 2022 Apr 21;: