Multiorgan and Vascular Tropism of SARS-CoV-2.
Fiche publication
Date publication
mars 2022
Journal
Viruses
Auteurs
Membres identifiés du Cancéropôle Est :
Dr KOSCINSKI Isabelle, Pr GAUCHOTTE Guillaume
Tous les auteurs :
Hartard C, Chaqroun A, Settembre N, Gauchotte G, Lefevre B, Marchand E, Mazeaud C, Nguyen DT, Martrille L, Koscinski I, Malikov S, Schvoerer E
Lien Pubmed
Résumé
Although the respiratory tract is the main target of SARS-CoV-2, other tissues and organs are permissive to the infection. In this report, we investigated this wide-spectrum tropism by studying the SARS-CoV-2 genetic intra-host variability in multiple tissues. The virological and histological investigation of multiple specimens from a post-mortem COVID-19 patient was performed. SARS-CoV-2 genome was detected in several tissues, including the lower respiratory system, cardio-vascular biopsies, stomach, pancreas, adrenal gland, mediastinal ganglion and testicles. Subgenomic RNA transcripts were also detected, in favor of an active viral replication, especially in testicles. Ultra-deep sequencing allowed us to highlight several SARS-CoV-2 mutations according to tissue distribution. More specifically, mutations of the spike protein, i.e., V341A (18.3%), E654 (44%) and H655R (30.8%), were detected in the inferior vena cava. SARS-CoV-2 variability can contribute to heterogeneous distributions of viral quasispecies, which may affect the COVID-19 pathogeny.
Mots clés
SARS-CoV-2, ultra-deep sequencing, viral quasispecies
Référence
Viruses. 2022 Mar 3;14(3):