Detection and dissemination of Toxoplasma gondii in experimentally infected calves, a single test does not tell the whole story.
Fiche publication
Date publication
janvier 2018
Journal
Parasites & vectors
Auteurs
Membres identifiés du Cancéropôle Est :
Pr VILLENA Isabelle
Tous les auteurs :
Burrells A, Taroda A, Opsteegh M, Schares G, Benavides J, Dam-Deisz C, Bartley PM, Chianini F, Villena I, van der Giessen J, Innes EA, Katzer F
Lien Pubmed
Résumé
Although the detection of Toxoplasma gondii in bovine tissues is rare, beef might be an important source of human infection. The use of molecular techniques, such as magnetic capture qPCR (MC-qPCR), in combination with the gold standard method for isolating the parasite (mouse bioassay), may increase the sensitivity of T. gondii detection in infected cattle. The risk of transmission of the parasite to humans from undercooked/raw beef is not fully known and further knowledge about the predilection sites of T. gondii within cattle is needed. In the current study, six Holstein Friesian calves (Bos taurus) were experimentally infected with 10 T. gondii oocysts of the M4 strain and, following euthanasia (42 dpi), pooled tissues were tested for presence of the parasite by mouse bioassay and MC-qPCR.
Mots clés
Beef, Cattle, Food safety, Magnetic capture, Mouse bioassay, Polymerase chain reaction, Toxoplasma gondii
Référence
Parasit Vectors. 2018 01 18;11(1):45