A rice tubulin tyrosine ligase like 12 regulates phospholipase D activity and tubulin synthesis.
Fiche publication
Date publication
mars 2022
Journal
Plant science : an international journal of experimental plant biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HEINTZ Dimitri
Tous les auteurs :
Zhang K, Shi W, Zheng X, Liu X, Wang L, Riemann M, Heintz D, Nick P
Lien Pubmed
Résumé
All plant α-tubulins encode a C-terminal tyrosine. An elusive tubulin tyrosine carboxypeptidase can cleave off, and a tubulin tyrosine ligase (TTL) re-ligate this tyrosine. The biological function of this cycle remains unclear but may correlate with microtubule stability. To get insight into the functional context of this phenomenon, we used cold-induced elimination of microtubules as experimental model. In previous work, we had analysed a rice TTL-like 12 (OsTTLL12), the only potential candidate of plant TTL. To follow the effect of OsTTLL12 upon microtubule responses in vivo, we expressed OsTTLL12-RFP into tobacco BY-2 cells stably overexpressing NtTUA3-GFP. We found that overexpression of OsTTLL12-RFP made microtubules disappear faster in response to cold stress, accompanied with more rapid Ca influx, culminating in reduced cold tolerance. Treatment with different butanols indicated that α-tubulin detyrosination/tyrosination differently interacts with phospholipase D (PLD) dependent signalling. In fact, rice PLDα1 decorated microtubules and increased detyrosinated α-tubulin. Unexpectedly, overexpression of the two proteins (OsTTLL12-RFP, NtTUA3-GFP) mutually regulated the accumulation of their transcripts, leading us to a model, where tubulin detyrosination feeds back upon tubulin transcripts and defines a subset of microtubules for interaction with PLD dependent stress signalling.
Mots clés
Detyrosination, Microtubule, Phospholipase D, Tubulin tyrosine carboxypeptidase, Tubulin tyrosine ligase like 12, Tyrosination
Référence
Plant Sci. 2022 Mar;316:111155