Reduced secretion and expression of gelatinase profile in Toxoplasma gondii-infected human monocytic cells.
Fiche publication
Date publication
juillet 2007
Journal
Biochemical and biophysical research communications
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BUACHE Emilie, Pr GILLERY Philippe, Pr VILLENA Isabelle
Tous les auteurs :
Buache E, Garnotel R, Aubert D, Gillery P, Villena I
Lien Pubmed
Résumé
The apicomplexan Toxoplasma gondii, an obligate intracellular parasite, can infect humans and a wide range of vertebrates. Following oral infection, the parasite invades tissues by crossing non-permissive biological barriers such as the placenta or the blood-brain barrier. But the molecular mechanisms underlying migration of T. gondii remain poorly characterized. The crossing of various basal membranes and infiltration into the extracellular matrix by T. gondii could involve matrix metalloproteinases (MMPs). We demonstrated a decrease in proMMP-2 and proMMP-9 secretion by THP-1 cells at 24 and 48h post invasion with regulation at the mRNA level throughout infection. This down regulation was associated with a decrease in TIMP-2 secretion and an inhibition of its expression. Moreover, results showed an activation of MT1-MMP; its expression was regulated after 6, 24, and 48h.
Mots clés
Animals, Cell Line, Cell Survival, Female, Gelatinases, biosynthesis, Gene Expression Regulation, Humans, Matrix Metalloproteinase 14, metabolism, Matrix Metalloproteinase 2, metabolism, Matrix Metalloproteinase 9, metabolism, Mice, Monocytes, metabolism, Time Factors, Tissue Inhibitor of Metalloproteinase-2, metabolism, Toxoplasma, metabolism
Référence
Biochem. Biophys. Res. Commun.. 2007 Jul;359(2):298-303