Molecular characterization and expression analysis of a P-glycoprotein homologue in Toxoplasma gondii.
Fiche publication
Date publication
janvier 2009
Journal
Molecular and biochemical parasitology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr TERRYN Christine, Pr VILLENA Isabelle
Tous les auteurs :
Schmid A, Sauvage V, Escotte-Binet S, Aubert D, Terryn C, Garnotel R, Villena I
Lien Pubmed
Résumé
ATP-binding cassette (ABC) transporters represent an important family of membrane proteins involved in drug resistance and other biological activities. The present study reports on the characterization of a P-glycoprotein (Pgp), TgABC.B1, in the protozoan parasite Toxoplasma gondii. The protein encoded by the TgABC.B1 gene displays the typical (TMD-NBD)2 structural organization of the "full" ABC transporter and shows significant identity and similarity with two apicomplexan Pgps; Pgh1 in Plasmodium falciparum and CpABC3 in Cryptosporidium parvum. The TgABC.B1 gene is a single copy gene transcribed into a full-length mRNA of 4.3kb and expressed as a protein of approximately 150kDa, which cellular localization revealed a membrane-associated labelling in tachyzoites. The TgABC.B1 gene is constitutively expressed in the three major T. gondii genotypes but demonstrated a higher expression in virulent type I, at both transcriptional and translational levels. Further characterization of this Pgp-like protein will increase our knowledge of the membrane transport system in this parasite and could result in the identification of a new therapeutic target in Toxoplasma.
Mots clés
ATP-Binding Cassette, Sub-Family B, Member 1, chemistry, Amino Acid Sequence, Animals, Female, Gene Expression, Humans, Mice, Models, Molecular, Molecular Sequence Data, Molecular Weight, Protozoan Proteins, chemistry, Sequence Alignment, Toxoplasma, chemistry, Toxoplasmosis, parasitology
Référence
Mol. Biochem. Parasitol.. 2009 Jan;163(1):54-60