Freestanding magnetic microtissues for tissue engineering applications.
Fiche publication
Date publication
décembre 2021
Journal
Advanced healthcare materials
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Santos LF, Patrício SG, Silva AS, Mano JF
Lien Pubmed
Résumé
A long-sought goal in tissue engineering is the development of tissues able to recapitulate the complex architecture of the native counterpart. Microtissues, by resembling the functional units of living structures, can be used to recreate tissues' architecture. Howbeit, microfabrication methodologies fail to reproduce cell-based tissues with uniform shape. At the macroscale, complex tissues were already produced by magnetic-tissue engineering using solely magnetized cells as building materials. The enhanced extracellular matrix deposition guaranties the conservation of tissues' architecture, leading to a successful cellular engraftment. Following the same rational, we now propose the combination of a versatile microfabrication-platform with magnetic-tissue engineering to generate robust micro-tissues with complex architecture for tissue engineering purposes. Small tissue units with circle, square and fiber-like shapes were designed with high fidelity acting as building blocks for engineering complex tissues. Notably, freestanding microtissues maintained their geometry after 7 days post-culturing, overcoming the challenges of microtissues fabrication. Lastly, the ability of microtissues in invading distinct tissue models while releasing trophic factors was substantiated in methacryloyl laminarin and platelet lysates hydrogels. By simply using cells as building units and such microfabrication-platform, we envisage the fabrication of complex multiscale and multifunctional tissues with clinical relevance, including for therapies or disease models. This article is protected by copyright. All rights reserved.
Mots clés
Building blocks, magnetic field, micropatterned surfaces, microtissues, tissue engineering
Référence
Adv Healthc Mater. 2021 Dec 18;:e2101532