Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.
Fiche publication
Date publication
novembre 2021
Journal
International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SOULIMANI Rachid
Tous les auteurs :
Soulimani R, Dicko A, Baudelaire E, Bouayed J
Lien Pubmed
Résumé
Anti-inflammatory effect of , , and were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) total plant materials obtained via hydroethanolic (HE) extraction. Further, phytochemical composition of the fine powders and HE extracts was determined by LC-PDA-ESI/MS analyses. Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05). For each of the plants studied, their superfine powdered fractions were more concentrated in phenolic contents than their HE extracts. Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.
Mots clés
Anti-inflammatory effect, Controlled differential sieving process, PBMCs, Phytochemical concentrations, Plant powders
Référence
Int J Vitam Nutr Res. 2021 Nov 24;: