Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses.
Fiche publication
Date publication
octobre 2021
Journal
Archives of virology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr PRETET Jean-Luc, Dr GUENAT David
Tous les auteurs :
Debernardi A, Jarassier W, Soret C, Mougin C, Alizon S, Bravo IG, Guenat D, Prétet JL, Lepiller Q
Lien Pubmed
Résumé
Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study was to develop a protocol for direct deep sequencing of high-risk (hr) HPV strains, based on the widely used commercial Hybrid Capture 2 (QIAGEN) test, without any additional probe design. This protocol was applied to 15 HPV-positive and two HPV-negative cervical samples or cell lines and validated at the genotype level by comparing the sequencing results to those obtained using a commercial genotyping kit. The performance of our protocol, presented in this proof-of-principle study, supports its use for accurate characterization of genetic variants of hrHPV.
Référence
Arch Virol. 2021 Oct 7;: