Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD.
Fiche publication
Date publication
août 2021
Journal
Diagnostics (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr POLETTE Myriam, Dr DORMOY Valerian
Tous les auteurs :
Ancel J, Belgacemi R, Diabasana Z, Perotin JM, Bonnomet A, Dewolf M, Launois C, Mulette P, Deslée G, Polette M, Dormoy V
Lien Pubmed
Résumé
Chronic obstructive pulmonary disease (COPD) is a frequent respiratory disease. However, its pathophysiology remains partially elucidated. Epithelial remodeling including alteration of the cilium is a major hallmark of COPD, but specific assessments of the cilium have been rarely investigated as a diagnostic tool in COPD. Here we explore the dysregulation of the ciliary function (ciliary beat frequency (CBF)) and differentiation (multiciliated cells formation in air-liquid interface cultures) of bronchial epithelial cells from COPD ( = 17) and non-COPD patients ( = 15). CBF was decreased by 30% in COPD (11.15 +/- 3.37 Hz vs. 7.89 +/- 3.39 Hz, = 0.037). Ciliary differentiation was altered during airway epithelial cell differentiation from COPD patients. While the number of multiciliated cells decreased ( < 0.005), the number of primary ciliated cells increased ( < 0.05) and primary cilia were shorter ( < 0.05). Altogether, we demonstrate that COPD can be considered as a ciliopathy through both primary non-motile cilia modifications (related to airway epithelial cell repair and remodeling) and motile cilia function impairment (associated with decrease sputum clearance and clinical respiratory symptoms). These observations encourage considering cilia-associated features in the complex COPD physiopathology and highlight the potential of cilia-derived biomarkers for diagnosis.
Mots clés
CBF, CiliOPD, airway epithelial cells, chronic obstructive pulmonary disease, cilia
Référence
Diagnostics (Basel). 2021 Aug 31;11(9):