Impact of optical clearing on ex vivo human skin optical properties characterized by spatially resolved multimodal spectroscopy.
Fiche publication
Date publication
septembre 2021
Journal
Journal of biophotonics
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BLONDEL Walter, Dr AMOUROUX Marine
Tous les auteurs :
Zaytsev SM, Amouroux M, Khairallah G, Bashkatov AN, Tuchin VV, Blondel W, Genina EA
Lien Pubmed
Résumé
A spatially resolved multimodal spectroscopic device was used on a two-layered "hybrid" model made of ex vivo skin and fluorescent gel to investigate the effect of skin optical clearing on the depth sensitivity of optical spectroscopy. Time kinetics of fluorescence and diffuse reflectance spectra were acquired in four experimental conditions: with Optical Clearing Agent (OCA) 1 made of polyethylene glycol 400 (PEG-400), propylene glycol (PG) and Sucrose; with OCA 2 made of PEG-400 and dimethyl sulfoxide (DMSO); with saline solution as control and a "dry" condition. An increase in the gel fluorescence back reflected intensity was measured after optical clearing. Effect of OCA 2 turned out to be stronger than that of OCA 1, possibly due to DMSO impact on the stratum corneum keratin conformation. Complementary experimental results showed increased light transmittance through the skin and confirmed that the improvement in the depth sensitivity of the multimodal spectroscopic approach is related not only to the dehydration and refractive indices matching due to optical clearing, but also to the mechanical compression of tissues caused by the application of the spectroscopic probe. This article is protected by copyright. All rights reserved.
Mots clés
depth resolution, diffuse reflectance, fluorescence, optical clearing, skin, spatially-resolved spectroscopy
Référence
J Biophotonics. 2021 Sep 3;:e202100202