Oxidative stress and brain mitochondria swelling induced by endosulfan and protective role of quercetin in rat.
Fiche publication
Date publication
mai 2015
Journal
Environmental science and pollution research international
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SOULIMANI Rachid
Tous les auteurs :
Lakroun Z, Kebieche M, Lahouel A, Zama D, Desor F, Soulimani R
Lien Pubmed
Résumé
The neurological damages resulted by endosulfan poisoning is not completely elucidated, especially in cellular organelles such as mitochondria. In the present study, the pro-oxidant effect of endosulfan on brain mitochondria was first investigated. Gavages of endosulfan into rats at the dose of 2 mg/kg induced oxidative stress in this organelle since it provokes a significant reduction of catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH) level. In addition, a significant increase in mitochondria swelling and malondialdehyde (MDA) levels were observed in neuronal mitochondria, indicating clearly an intense peroxidation within mitochondria. Second, the protective effect of quercetin (QE) (10 mg/kg) against endosulfan-induced oxidative stress in mitochondria was also assessed. Indeed, the pretreatment of rats with QE protects brain mitochondria from oxidative stress, lipid peroxidation, and mitochondria swelling induced by endosulfan. The activities of antioxidant enzymes and the mitochondrial content of GSH and MDA were returned to control values. Thus, although endosulfan can have neurotoxic effects in brain rats, this toxicity can be prevented by quercetin.
Mots clés
Animals, Antioxidants, pharmacology, Brain, drug effects, Catalase, metabolism, Endosulfan, toxicity, Glutathione, metabolism, Glutathione Peroxidase, metabolism, Insecticides, toxicity, Lipid Peroxidation, drug effects, Male, Malondialdehyde, Mitochondria, Oxidative Stress, drug effects, Quercetin, pharmacology, Rats, Rats, Wistar, Reactive Oxygen Species, pharmacology, Superoxide Dismutase, metabolism
Référence
Environ Sci Pollut Res Int. 2015 May;22(10):7776-81