Electrically Switchable Nanolever Technology for the Screening of Metal-Chelating Peptides in Hydrolysates.
Fiche publication
Date publication
juillet 2021
Journal
Journal of agricultural and food chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BOSCHI-MULLER Sandrine
Tous les auteurs :
El Hajj S, Sepúlveda Rincón CT, Girardet JM, Cakir-Kiefer C, Stefan L, Zapata Montoya JE, Boschi-Muller S, Gaucher C, Canabady-Rochelle L
Lien Pubmed
Résumé
Metal-chelating peptides (MCP) are considered as indirect antioxidants due to their capacity to inhibit radical chain reaction and oxidation. Here, we propose a new proof of concept for the screening of MCPs present in protein hydrolysates for valorizing their antioxidant properties by using the emerging time-resolved molecular dynamics technology, switchSENSE. This method unveils possible interactions between MCPs and immobilized nickel ions using fluorescence and electro-switchable DNA chips. The switchSENSE method was first set up on synthetic peptides known for their metal-chelating properties. Then, it was applied to soy and tilapia viscera protein hydrolysates. Their Cu-chelation capacity was, in addition, determined by UV-visible spectrophotometry as a reference method. The switchSENSE method has displayed a high sensitivity to evidence the presence of MCPs in both hydrolysates. Hence, we demonstrate for the first time that this newly introduced technology is a convenient methodology to screen protein hydrolysates in order to determine the presence of MCPs before launching time-consuming separations.
Mots clés
electro-switchable chip, hydrolysate, metal-chelating peptide, soy protein, tilapia protein
Référence
J Agric Food Chem. 2021 Jul 29;: