Successful retrieval of human papillomavirus DNA after a 4.5 year storage on FTA elute cards.
Fiche publication
Date publication
juin 2021
Journal
Journal of virological methods
Auteurs
Membres identifiés du Cancéropôle Est :
Dr AVEROUS Gerlinde, Pr BALDAUF Jean-Jacques, Dr FENDER Muriel, Pr PRETET Jean-Luc
Tous les auteurs :
Prétet JL, Baraquin A, Soret C, Rousselot J, Averous G, Fender M, Mougin C, Baldauf JJ
Lien Pubmed
Résumé
Efficient primary (vaccination) and secondary (screening) prevention strategies have the potential to eliminate cervical cancer worldwide. In this context, surveillance of HPV infections remains mandatory to assess the efficacy and the impact of screening and vaccination policies. Therefore there is a need to safely store cervical samples to conduct long-term studies in vaccinated and non-vaccinated subjects. Up-dated data on cervical specimen preservation on FTA® cards indicate that HPV DNA can be safely retrieved after 54 months of storage. A concordance of 97% was achieved between HPV genotypes detected in initial cervical samples and on FTA® cards 4.5 years later. Even if a drop in HPV viral loads was observed in some cases at 4.5 years, using FTA® cards for safe and long-term storage of cervical samples represents an interesting option.
Mots clés
DNA preservation, FTA, HPV, Papillomavirus, biobanking
Référence
J Virol Methods. 2021 Jun 24;:114218