Reduced levels of prostaglandin I synthase: a distinctive feature of the cancer-free trichothiodystrophy.
Fiche publication
Date publication
juin 2021
Journal
Proceedings of the National Academy of Sciences of the United States of America
Auteurs
Membres identifiés du Cancéropôle Est :
Dr COMPE Emmanuel
Tous les auteurs :
Lombardi A, Arseni L, Carriero R, Compe E, Botta E, Ferri D, Uggè M, Biamonti G, Peverali FA, Bione S, Orioli D
Lien Pubmed
Résumé
The cancer-free photosensitive trichothiodystrophy (PS-TTD) and the cancer-prone xeroderma pigmentosum (XP) are rare monogenic disorders that can arise from mutations in the same genes, namely or Both XPD and XPB proteins belong to the 10-subunit complex transcription factor IIH (TFIIH) that plays a key role in transcription and nucleotide excision repair, the DNA repair pathway devoted to the removal of ultraviolet-induced DNA lesions. Compelling evidence suggests that mutations affecting the DNA repair activity of TFIIH are responsible for the pathological features of XP, whereas those also impairing transcription give rise to TTD. By adopting a relatives-based whole transcriptome sequencing approach followed by specific gene expression profiling in primary fibroblasts from a large cohort of TTD or XP cases with mutations in gene, we identify the expression alterations specific for TTD primary dermal fibroblasts. While most of these transcription deregulations do not impact on the protein level, very low amounts of prostaglandin I synthase (PTGIS) are found in TTD cells. PTGIS catalyzes the last step of prostaglandin I synthesis, a potent vasodilator and inhibitor of platelet aggregation. Its reduction characterizes all TTD cases so far investigated, both the PS-TTD with mutations in TFIIH coding genes as well as the nonphotosensitive (NPS)-TTD. A severe impairment of TFIIH and RNA polymerase II recruitment on the promoter is found in TTD but not in XP cells. Thus, PTGIS represents a biomarker that combines all PS- and NPS-TTD cases and distinguishes them from XP.
Mots clés
NER-defective disorders, PTGIS, TFIIH transcription
Référence
Proc Natl Acad Sci U S A. 2021 Jun 29;118(26):