Impact of GG on the Emulsion Stability of Raw Milk.
Fiche publication
Date publication
mai 2021
Journal
Foods (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DUMAS Dominique
Tous les auteurs :
Dos Santos Morais R, Louvet N, Borges F, Dumas D, Cvetkovska-Ben Mohamed L, Barrau S, Scher J, Gaiani C, Burgain J
Lien Pubmed
Résumé
Lactic acid bacteria (LAB) have been studied for several decades to understand and determine their mechanism and interaction within the matrix into which they are introduced. This study aimed to determine the spatial distribution of GG (LGG) in a dairy matrix and to decipher its behaviour towards milk components, especially fat globules. Two strains of this widely studied bacterium with expected probiotic effects were used: LGG WT with pili on the cell surface and its pili-depleted mutant-LGG Δ-in order to determine the involvement of these filamentous proteins. In this work, it was shown that LGG Δ was able to limit creaming with a greater impact than the wild-type counterpart. Moreover, confocal imaging evidenced a preferential microbial distribution as aggregates for LGG WT, while the pili-depleted strain tended to be homogenously distributed and found as individual chains. The observed differences in creaming are attributed to the indirect implication of SpaCBA pili. Indeed, the bacteria-to-bacteria interaction surpassed the bacteria-to-matrix interaction, reducing the bacterial surface exposed to raw milk. Conversely, LGG Δ may form a physical barrier responsible for preventing milk fat globules from rising to the surface.
Mots clés
Lacticaseibacillus rhamnosus GG (LGG), confocal laser scanning microscopy (CLSM), creaming, fat globule, flocculation, lactic acid bacteria, microbial distribution, raw milk
Référence
Foods. 2021 May 1;10(5):