Molecular organization in hydroperoxidized POPC bilayers.
Fiche publication
Date publication
mai 2021
Journal
Biochimica et biophysica acta. Biomembranes
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves, Dr KLYMCHENKO Andrey
Tous les auteurs :
Junqueira H, Schroder AP, Thalmann F, Klymchenko A, Mély Y, Baptista MS, Marques CM
Lien Pubmed
Résumé
Lipid hydroperoxides are the primary reaction products of lipid oxidation, a natural outcome of life under oxygen. While playing a major role in cell metabolism, the microscopic origins of the effects of lipid hydroperoxidation on biomembranes remain elusive. Here we probe the polar structure of partially to fully hydroperoxidized bilayers of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) by a combination of environment-sensitive fluorescent probes and coarse-grained Martini numerical simulations. We find that the inserted organic hydroperoxide group -OOH migrates preferentially to the surface for bilayers with small fractions of hydroperoxidized lipids, but populates also significantly the bilayer interior for larger fractions. Our findings suggest that by modifying the intimate polarity of biomembranes, lipid peroxidation will have a significant impact on the activity of transmembrane proteins and on the bio-medical efficiency of membrane active molecules such as cell-penetrating and antimicrobial peptides.
Mots clés
MARTINI simulations, bilayer structure, hydroperoxide localization, hydroxyflavone probes, phospholipid hydroperoxidation
Référence
Biochim Biophys Acta Biomembr. 2021 May 27;:183659