Forever Young: Structural Stability of Telomeric Guanine-Quadruplexes in Presence of Oxidative DNA Lesions.
Fiche publication
Date publication
avril 2021
Journal
Chemistry (Weinheim an der Bergstrasse, Germany)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GRANDEMANGE Stéphanie
Tous les auteurs :
Miclot T, Corbier C, Terenzi A, Hognon C, Grandemange S, Barone G, Monari A
Lien Pubmed
Résumé
Human telomeric DNA,in G-quadruplex (G4) conformation, is characterized by a remarkable structural stability that confers it the capacity to resist to oxidative stress producing one or even clustered 8-oxoguanine (8oxoG) lesions. We present a combined experimental/computational investigation, by using circular dichroism in aqueous solutions, cellular immunofluorescence assays and molecular dynamics simulations, that identifies the crucial role of the stability of G4s to oxidative lesions, related also to their biological role as inhibitors of telomerase, an enzyme overexpressed in most cancers associated to oxidative stress.
Mots clés
Guanine quadruplexes, Molecular dynamics, electronic circular dichroism, immunofluorescence, oxidative DNA lesions
Référence
Chemistry. 2021 Apr 19;: