Ultrabright Green-Emitting Nanoemulsions Based on Natural Lipids-BODIPY Conjugates.
Fiche publication
Date publication
mars 2021
Journal
Nanomaterials (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr KLYMCHENKO Andrey
Tous les auteurs :
Wang X, Bou S, Klymchenko AS, Anton N, Collot M
Lien Pubmed
Résumé
Nanoemulsions (NEs) are water-dispersed oil droplets that constitute stealth biocompatible nanomaterials. NEs can reach an impressive degree of fluorescent brightness owing to their oily core that can encapsulate a large number of fluorophores on the condition the latter are sufficiently hydrophobic and oil-soluble. BODIPYs are among the brightest green emitting fluorophores and as neutral molecules possess high lipophilicity. Herein, we synthesized three different natural lipid-BODIPY conjugates by esterification of an acidic BODIPY by natural lipids, namely: α-tocopherol (vitamin E), cholesterol, and stearyl alcohol. The new BODIPY conjugates were characterized in solvents and oils before being encapsulated in NEs at various concentrations. The physical (size, stability over time, leakage) and photophysical properties (absorption and emission wavelength, brightness, photostability) are reported and showed that the nature of the lipid anchor and the nature of the oil used for emulsification greatly influence the properties of the bright NEs.
Mots clés
BODIPY, brightness, fluorescence, fluorescence correlation spectroscopy, nanoemulsions
Référence
Nanomaterials (Basel). 2021 Mar 23;11(3):