Stem-Loop qRT-PCR-Based Quantification of miRNAs.
Fiche publication
Date publication
janvier 2021
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr REDERSTORFF Mathieu
Tous les auteurs :
Abel Y, Rederstorff M
Lien Pubmed
Résumé
Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.
Mots clés
MicroRNA, Real-time PCR quantification, Small non-coding RNA, Stem-loop primers
Référence
Methods Mol Biol. 2021 ;2300:59-64