Interaction of implant infection-related commensal bacteria with mesenchymal stem cells: A comparison between Cutibacterium acnes and Staphylococcus aureus.
Fiche publication
Date publication
février 2021
Journal
FEMS microbiology letters
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie, Dr KERDJOUDJ Halima
Tous les auteurs :
El-Mahdy TS, Mongaret C, Varin-Simon J, Lamret F, Vernet-Garnier V, Rammal H, Mauprivez C, Kerdjoudj H, Gangloff SC, Reffuveille F
Lien Pubmed
Résumé
Staphylococcus aureus and Cutibacterium acnes are involved in several tissue infections and can encounter mesenchymal stem cells (MSCs) during their role in tissue regenerative process. C. acnes and S. aureus internalization by three types of MSCs derived from bone marrow, dental pulp and Wharton's jelly; and bacterial biofilm production were compared. Internalization rates ranged between 1.7%-6.3% and 0.8%-2.7% for C. acnes and S. aureus, respectively. While C. acnes strains exhibited limited cytotoxic effect on MSCs, S. aureus were more virulent with marked effect starting after only three hours of interaction. Both bacteria were able to produce biofilms with respectively aggregated and monolayered structures for C. acnes and S. aureus. The increase in C. acnes capacity to develop biofilm following MSCs' internalization was not linked to the significant increase in number of live bacteria, except for bone marrow-MSCs/C. acnes CIP 53.117 with 79% live bacteria compared to the 36% before internalization. On the other hand, internalization of S. aureus had no impact on its ability to form biofilms composed mainly of living bacteria. The present study underlined the complexity of MSCs-bacteria cross-interaction and brought insights into understanding the MSCs behaviour in response to bacterial infection in tissue regeneration context.
Mots clés
Cutibacterium acnes , Staphylococcus aureus , Mesenchymal stem cells, biofilm, internalization
Référence
FEMS Microbiol Lett. 2021 Feb 13;: