Live-cell imaging of the nucleolus and mapping mitochondrial viscosity with a dual function fluorescent probe.
Fiche publication
Date publication
février 2021
Journal
Organic & biomolecular chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves, Dr KLYMCHENKO Andrey
Tous les auteurs :
Mukherjee T, Soppina V, Ludovic R, Mély Y, Klymchenko AS, Collot M, Kanvah S
Lien Pubmed
Résumé
Visualization of sub-cellular organelles allows the determination of various cellular processes and the underlying mechanisms. Herein, we report a fluorescent probe, bearing push-pull substituents emitting at 600 nm and its application in cellular imaging. The probe shows dual imaging of mitochondria and nucleoli and maps mitochondrial viscosity in live cells under various physiological variations and show minimum cytotoxicity. Nucleolar staining is confirmed by RNAase digestion.
Référence
Org Biomol Chem. 2021 Feb 8;: