Real-Time Imaging of Bacteria/Osteoblast Dynamic Coculture on Bone Implant Material in an in Vitro Postoperative Contamination Model.
Fiche publication
Date publication
juillet 2019
Journal
ACS biomaterials science & engineering
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ANSELME Karine
Tous les auteurs :
Prévost V, Anselme K, Gallet O, Hindié M, Petithory T, Valentin J, Veuillet M, Ploux L
Lien Pubmed
Résumé
Biomedical implants are an important part of evolving modern medicine but have a potential drawback in the form of postoperative pathogenic infection. Accordingly, the "race for surface" combat between invasive bacteria and host cells determines the fate of implants. Hence, proper in vitro systems are required to assess effective strategies to avoid infection. In this study, we developed a real time observation model, mimicking postoperative contamination, designed to follow proliferation on a titanium surface occupied by human osteoblastic progenitor cells (STRO). This model allowed us to monitor invasion of human cells on titanium surfaces coated and uncoated with fibronectin. We showed that the surface colonization of bacteria is significantly enhanced on fibronectin coated surfaces irrespective of whether areas were uncovered or covered with human cells. We further revealed that bacterial colonization of the titanium surfaces is enhanced in coculture with STRO cells. Finally, this coculture system provides a comprehensive system to describe in vitro and in situ bacterial and human cells and their localization but also to target biological mechanisms involved in adhesion as well as in interactions with surfaces, thanks to fluorescent labeling. This system is thus an efficient method for studies related to the design and function of new biomaterials.
Mots clés
bacteria/eukaryotic cells coculture, biomaterials, confocal fluorescence microscopy, fibronectin, real-time imaging
Référence
ACS Biomater Sci Eng. 2019 Jul 8;5(7):3260-3269