Transfection of Small Noncoding RNAs into Arabidopsis thaliana Protoplasts.
Fiche publication
Date publication
janvier 2020
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MARÉCHAL-DROUARD Laurence
Tous les auteurs :
Lalande S, Chery M, Ubrig E, Hummel G, Kubina J, Geldreich A, Drouard L
Lien Pubmed
Résumé
Polyethylene glycol transfection of plant protoplasts represents an efficient method to incorporate foreign DNA and study transient gene expression. Here, we describe an optimized protocol to deliver small noncoding RNAs into Arabidopsis thaliana protoplasts. An example of application is provided by demonstrating the incorporation of a 20 nt long small noncoding RNA deriving from the 5' extremity of an A. thaliana cytosolic alanine tRNA into freshly isolated protoplasts.
Mots clés
Northern blot, Protoplasts, Small noncoding RNAs, Transfection, tRNA-derived fragments
Référence
Methods Mol Biol. 2020 ;2166:413-429