Gene bashing of locus identifies genomic regions important for rectal cell expression and rescue of its mutant lethality.
Fiche publication
Date publication
décembre 2020
Journal
microPublication biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr JARRIAULT Sophie
Tous les auteurs :
Ahier A, Suman SK, Jarriault S
Lien Pubmed
Résumé
Strong loss-of-function or null mutants can sometimes lead to a penetrant early lethality, impairing the study of these genes' function. This is the case for the null mutant, which exhibits 100% penetrant lethality. Here, we describe how we used gene bashing to identify distinct regulatory regions in the locus. This allowed us to generate a null strain that is viable and still displays mutant Y-to-PDA transdifferentiation phenotype. Such strategy can be applied to many other mutants impacting viability.
Référence
MicroPubl Biol. 2020 Dec 21;2020: