Sedimentation Velocity Methods for the Characterization of Protein Heterogeneity and Protein Affinity Interactions.
Fiche publication
Date publication
janvier 2021
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BIRCK Catherine
Tous les auteurs :
Ebel C, Birck C
Lien Pubmed
Résumé
Sedimentation velocity analytical ultracentrifugation is a powerful and versatile tool for the characterization of proteins and macromolecular complexes in solution. The direct modeling of the sedimentation process using modern computational strategies allows among others to assess the homogeneity/heterogeneity state of protein samples and to characterize protein associations. In this chapter, we will provide theoretical backgrounds and protocols to analyze the size distribution of protein samples and to determine the affinity of protein-protein hetero-associations.
Mots clés
Analytical ultracentrifugation, Hetero-association, Protein heterogeneity, Protein interaction, Sedimentation coefficient, Sedimentation velocity
Référence
Methods Mol Biol. 2021 ;2247:155-171