Hands on Native Mass Spectrometry Analysis of Multi-protein Complexes.
Fiche publication
Date publication
janvier 2021
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CIANFERANI Sarah
Tous les auteurs :
Erb S, Cianférani S, Marcoux J
Lien Pubmed
Résumé
By maintaining intact multi-protein complexes in the gas-phase, native mass spectrometry provides their molecular weight with very good accuracy compared to other methods (typically native PAGE or SEC-MALS) (Marcoux and Robinson, Structure 21:1541-1550, 2013). Besides, heterogeneous samples, in terms of both oligomeric states and ligand-bound species can be fully characterized. Here we thoroughly describe the analysis of several oligomeric protein complexes ranging from a 16 = kDa dimer to a 801-kDa tetradecameric complex on different instrumental setups.
Mots clés
Noncovalent mass spectrometry, Oligomeric states, Stoichiometry, Structural mass spectrometry, Subcomplexes
Référence
Methods Mol Biol. 2021 ;2247:173-191