Interactions and effects of BSA-functionalized single-walled carbon nanotubes on different cell lines.
Fiche publication
Date publication
avril 2016
Journal
Nanotechnology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BIANCO Alberto
Tous les auteurs :
Muzi L, Tardani F, La Mesa C, Bonincontro A, Bianco A, Risuleo G
Lien Pubmed
Résumé
Functionalized carbon nanotubes (CNTs) have shown great promise in several biomedical contexts, spanning from drug delivery to tissue regeneration. Thanks to their unique size-related properties, single-walled CNTs (SWCNTs) are particularly interesting in these fields. However, their use in nanomedicine requires a clear demonstration of their safety in terms of tissue damage, toxicity and pro-inflammatory response. Thus, a better understanding of the cytotoxicity mechanisms, the cellular interactions and the effects that these materials have on cell survival and on biological membranes is an important first step for an appropriate assessment of their biocompatibility. In this study we show how bovine serum albumin (BSA) is able to generate homogeneous and stable dispersions of SWCNTs (BSA-CNTs), suggesting their possible use in the biomedical field. On the other hand, this study wishes to shed more light on the impact and the interactions of protein-stabilized SWCNTs with two different cell types exploiting multidisciplinary techniques. We show that BSA-CNTs are efficiently taken up by cells. We also attempt to describe the effect that the interaction with cells has on the dielectric characteristics of the plasma membrane and ion flux using electrorotation. We then focus on the BSA-CNTs' acute toxicity using different cellular models. The novel aspect of this work is the evaluation of the membrane alterations that have been poorly investigated to date.
Mots clés
Animals, Apoptosis, drug effects, Cell Line, Cell Survival, drug effects, HEK293 Cells, Humans, Mice, Nanotubes, Carbon, chemistry, Serum Albumin, Bovine, chemistry
Référence
Nanotechnology. 2016 Apr 15;27(15):155704