Bovine Chromaffin Cells: Culture and Fluorescence Assay for Secretion.
Fiche publication
Date publication
janvier 2021
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BADER Marie-France, Dr GASMAN Stéphane, Dr CHASSEROT-GOLAZ Sylvette, Dr VITALE Nicolas
Tous les auteurs :
Thahouly T, Tanguy E, Raherindratsara J, Bader MF, Chasserot-Golaz S, Gasman S, Vitale N
Lien Pubmed
Résumé
Over the last four decades, chromaffin cells originating from the adrenal medulla have been probably one of the most popular cell models to study neurosecretion at the molecular level. Accordingly, numerous seminal discoveries in the field, including the characterization of role of the cytoskeleton, fusogenic lipids, and soluble N-ethylmaleimide-sensitivefactor attachment protein receptor (SNARE) proteins, have been made using this model. In this chapter, we describe a standard method currently used to isolate and culture bovine chromaffin cells, and we illustrate a catecholamine secretion assay based on the successive transformation of adrenaline into adrenochrome and adrenolutine for fluorescence measurements. We also provide some guidelines for efficient cell recovery and for the use of this assay in the laboratory.
Mots clés
Catecholamine secretion, Chromaffin cells, Fluorescence assay
Référence
Methods Mol Biol. 2021 ;2233:169-179