Comparison of double-locus sequence typing (DLST) and multilocus sequence typing (MLST) for the investigation of Pseudomonas aeruginosa populations.
Fiche publication
Date publication
août 2015
Journal
Diagnostic microbiology and infectious disease
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HOCQUET Didier
Tous les auteurs :
Cholley P, Stojanov M, Hocquet D, Thouverez M, Bertrand X, Blanc DS
Lien Pubmed
Résumé
Reliable molecular typing methods are necessary to investigate the epidemiology of bacterial pathogens. Reference methods such as multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) are costly and time consuming. Here, we compared our newly developed double-locus sequence typing (DLST) method for Pseudomonas aeruginosa to MLST and PFGE on a collection of 281 isolates. DLST was as discriminatory as MLST and was able to recognize "high-risk" epidemic clones. Both methods were highly congruent. Not surprisingly, a higher discriminatory power was observed with PFGE. In conclusion, being a simple method (single-strand sequencing of only 2 loci), DLST is valuable as a first-line typing tool for epidemiological investigations of P. aeruginosa. Coupled to a more discriminant method like PFGE or whole genome sequencing, it might represent an efficient typing strategy to investigate or prevent outbreaks.
Mots clés
MLST, Molecular typing, PFGE, Pseudomonas aeruginosa, Pulsed field gel electrophoresis, Sequence typing
Référence
Diagn. Microbiol. Infect. Dis.. 2015 Aug;82(4):274-7